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Figure 6 | Breast Cancer Research

Figure 6

From: Targeting of the non-mutated tumor antigen HER2/neu to mature dendritic cells induces an integrated immune response that protects against breast cancer in mice

Figure 6

Induction of anti-HER2/neu humoral immunity. FVB/N mice were vaccinated with DEC-HER2 or Ctrl Ig-HER2 with poly IC as the adjuvant. Sera were collected 2 weeks after the boost. (A) Titers of anti-HER2 IgG were quantified by enzyme-linked immunosorbent assay as described in Materials and methods. (B, C) Binding of HER2/neu-expressing tumor cells with immune sera measured by flow cytometry. Sera were diluted 1:100, 1:400, or 1:1,600 and incubated with HER2-expressing E0771/E2 (B) or rat neu-expressing NT2.5 (C) tumor cells for 15 minutes at 4°C. Binding of antibody to tumor cells was detected by staining with anti-mouse IgG-PE antibody and fluorescence-activated cell sorting analysis. Mean fluorescence indices (MFIs) are shown. **P < 0.01, ***P < 0.001. HER, human epidermal growth factor receptor; Ig, immunoglobulin; NS, not statistically significant; PE, phycoerythrin; poly IC, polyinosinic/polycytidylic acid.

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Breast Cancer Research

ISSN: 1465-542X

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