Figure 3

Focal adhesion kinase (FAK)-deleted cells do not contribute to lung metastasis in the mouse mammary tumour virus (MMTV)-Cre/NDL2-5 mouse model. (a) Bar graphs indicate the percentage of lung metastases for FAK^wt/wt, FAK^flox/wt and FAK^flox/flox/MMTV-Cre/-NDL2-5 mice and in (b) the average number of metastatic foci per lung lobe. Error bars represent the standard error of the mean (SEM). No statistically significant differences were observed (unpaired Student t-test). (c) Representative sections of X-gal-stained lung lobes from FAK^wt/wt (left) (n = 7) and FAK^flox/flox (right) (n = 8)/MMTV-Cre/NDL2-5/GTRosa26 mice bearing late-stage tumours. Arrows (lower) indicate lung lesions lacking positive X-gal staining. Scale bars: 500 μm. (d) Bar graphs indicate the percentage of X-gal-positive lung metastases in FAK^wt/wt (75% of metastases, n = 7) and FAK^flox/flox/MMTV-Cre/-NDL2-5 mice (0% of metastases, n = 8).