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Figure 3 | Breast Cancer Research

Figure 3

From: The growth response to androgen receptor signaling in ERα-negative human breast cells is dependent on p21 and mediated by MAPK activation

Figure 3

Androgen Receptor In Breast Epithelium (ARIBE) cells stimulate mitogen-activated protein kinase (MAPK) signaling upon androgen receptor (AR) ligand binding. Control cells (MCF-10A and 10A plus vector) and ARIBE cells were cultured under normal propagation conditions in the presence or absence of 20 ng/ml epidermal growth factor (EGF), treated with either vehicle or 1 nmol/l R1881 for 48 hours, and then used to make lysates for western blotting as described in Methods. Blots were probed for phosphorylated extracellular signal-regulated kinase (ERK) (Thr-202/Tyr-204) and total ERK. GAPDH antibody was used as loading control.

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Breast Cancer Research

ISSN: 1465-542X

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