Figure 5

Let-7a regulates cell migration and invasion of MDA-MB-231 and MCF-7 cell lines, dependent on CCR-7-CCL21 signaling. (a) mRNA expression of CCL21 and CCR7, and protein expression of CCR7 were detected with RT-PCR and Western blot analysis after transfection with scRNA, synthetic let-7a, and CCL21 siRNA in MDA-MB-231 cells, respectively. β-actin was used as a normalization control. (b) mRNA expression of CCL21 and CCR7, and protein expression of CCR7 were detected with RT-PCR and Western blot analysis after transfection with scRNA, anti-let-7a, and CCL21 siRNA in MCF-7 cells, respectively. β-actin was used as a normalization control. (c) After transfection with scRNA, synthetic let-7a, and CCL21 siRNA into MDA-MB-231cells, cell-migration assays and cell-invasion assays were performed (*^, **P < 0.01 versus control group). (d) After transfection with scRNA, synthetic anti-let-7a, and CCL21 siRNA into McF-7 cells, cell-migration assays and cell-invasion assays were performed (*^, **P < 0.01 versus control group). The assays were described in Material and Methods, and the results of (c) and (d) are presented as a histogram. All experiments were performed in triplicate independently.