Use of reverse transcription quantitative PCR (RTqPCR) to assess differences in the expression of Tumor-necrosis factor (TNF-α)-related genes between MCF-7
cells (a) and between A2780 and A2780
cells (b). For genes in which qPCR confirmed the changes in gene expression identified by cDNA microarray analysis, representative amplification plots are shown. S28 was used as the reference gene.