Lack of EGFR-activating mutations in European patients with triple-negative breast cancer could emphasise geographic and ethnic variations in breast cancer mutation profiles

Table 1 Primer sequences^a

GenBank accession number	Gene name and primer	Sequence 5'-3'	Size (nt)	GC (%)	Amplicon (bp)	Annealing temperature
NM_005228	EGFR Exon 19 forward	CGTCTTCCTTCTCTCTCTGTCATAGG	26	50%	182	58°C
	EGFR Exon 19 reverse	AAAAGGTGGGCCTGAGGTTC	20	55%
	EGFR Exon 21 forward	TTGGAGGACCGTCGCTTG	18	61.1%	164	58°C
	EGFR Exon 21 reverse	ACCTAAAGCCACCTCCTTACTTTG	24	45.8%

^aEGFR = epidermal growth factor receptor; GC = percentage of guanines and cytosines in the primers' sequence; nt = nucleotide.

ISSN: 1465-542X