Figure 2
From: CHEK2 contribution to hereditary breast cancer in non-BRCAfamilies
Kinase activity of recombinant Flag-CHEK2 protein. Total protein extract (1.5 μg) was tested for the ability to phosphorylate a fluorescent substrate. The slope of the resulting curve represents Flag-CHEK2 kinase activity. The slope of the wild-type (WT) Flag-CHEK2 kinase activity curve was normalized to 1. Nontransformed protein extracts (NT) and mutant c.1100delC served as controls. Each point represents an average of five measurements performed in triplicate.