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Figure 8 | Breast Cancer Research

Figure 8

From: ARTEMIN synergizes with TWIST1 to promote metastasis and poor survival outcome in patients with ER negative mammary carcinoma

Figure 8

ARTEMIN (ARTN) activates Protein kinase B (AKT) to increase TWIST1 expression in estrogen receptor (ER) negative mammary carcinoma (ER-MC). (a) Western blot analyses for phospho AKT (pAKT-Ser473) in BT549 and MDA-MB-231 cells with forced expression or depletion of ARTN. β-ACTIN was used as loading control for cell lysates. The sizes of detected protein bands in kiloDalton (kDa) are shown on the right. (b) Western blot was used to determine pAKT, AKT and TWIST1 protein levels in BT549-VEC and -ARTN cells ± AKT Inhibitor IV (10 μM). β-ACTIN was used as loading control. (c) Colony formation in soft agar. (d) Cell growth (number) in 3D matrigel. (e) Cell migration, and (f) Cell invasion was determined for BT549-VEC and -ARTN cells ± AKT inhibitor IV. Dimethyl sulfoxide (DMSO)-treated vector (VEC) cells are presented as 100%. *, P < 0.05; **, P < 0.01.

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Breast Cancer Research

ISSN: 1465-542X

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