Figure 4

Accumulation of fibrous tissue in the mammary gland of Ncadk.i. and Ncadk.i.;p53 mice. Trichrome staining of paraffin sections was used to visualize the accumulation of fibrous tissue in (A) Ncadk.i. and (B) Ncadk.i.;p53 mammary glands in the second and tenth lactation cycles. The pronounced deposit of collagen fibers in the second lactation cycle became markedly increased in females after ten lactation cycles. Mammary glands from Ecadk.o. and Ecadk.o.;p53 females showed only minor signs of fibrosis in comparison to the controls. Scale bar: 100 μm. Ecadk.o.;p53, WAP::Cre;Ecad^fl/fl;p53^fl/+; Ecadk.o., WAP::Cre;Ecad^fl/fl; Ncadk.i., WAP::Cre;Ecad^Ncad/fl; Ncadk.i.;p53, WAP::Cre;Ecad^Ncad/fl;p53^fl/+.