Figure 2

The effects of BRCA2 deletion in phenotypic context. (a) Deletion at the BRCA2 locus measured by segmented aCGH log₂ ratios (left) and TaqMan qPCR (right) in relation to phenotype. (b) Example profiles derived from two BRCA2 tumors (a19861102, a128049) of the triple-negative subtype analyzed by using 385 K aCGH (about one probe for each 7 kb). DNA gains or deletions are represented on the y-axis as black bars toward positive or negative values, respectively, in order of the chromosomal location from p- to q-arm (left to right). Focused views of chromosome 13 are displayed below each of the two profiles showing log₂ ratios (gray dots) on y-axis by nucleotide position on x-axis from p-arm to q-arm (left to right) with cytobands and the BRCA2 gene locus at 13q13.1 indicated as shown at the bottom of each plot. (c) S phase, (d) Ki-67, and (e) Topo IIa in relation to changes at the BRCA2 gene locus, affecting the wild-type allele, in luminal- (left panel, gray circles) and triple-negative phenotypes (right panel, black triangles).