Figure 4

Dietary fat stimulates the lung and liver metastasis of 4T1 cells in BALB/c mice. Mice were fed, injected with 4T1 cells, and then killed as described in Figure 1c. The lungs and livers were excised from mice and fixed in Bouin solution. The numbers of tumor nodules in the lung and liver was counted, and the total tumor volumes were estimated. (a, d) Photographs of the lungs and livers, which were representative of 15 animals, are shown. Whitish tumor lesions can be observed on the surfaces of the lungs and livers. (b, e) The numbers of tumor nodules in the lung and liver. (c, f) The volume of tumor nodules in the lung and liver. Each bar represents the mean ± SEM (n = 15). *Significantly different from the control group, P < 0.05.