SEPT9 isoforms alter migratory properties of MCF7. (A) Transwell migration assay comparing MCF7 clones, each expressing a different GFP-SEPT9 isoform fusion construct. Plotting reflects the average number of migrated cells from two biological replicate assays conducted for each isoform. (B) Cell cluster size distribution for each MCF7 clone. The same gray scale is used in (A) and (B) to represent MCF7 and each isoform construct.