Figure 5

SEPT9 isoforms localize in different cellular compartments. Fluorescence microscopy of MCF7 clones expressing individual GFP-SEPT9 isoforms (gray scale). Expression of GFP-SEPT9 isoform constructs was quantified and plotted as a ratio of intensities measured outside the region occupied by the nucleus to measurements within the nucleus (bottom right panel). Average ratios located above the dotted line indicate cytoplasmic expression and those below the line indicate nuclear expression.