Figure 2From: Epithelial to mesenchymal transition markers expressed in circulating tumour cells of early and metastatic breast cancer patientsTwist and vimentin expression in CTCs of early and metastatic breast cancer patients. (A) Graph I: Quantification of 25 early and 25 metastatic breast cancer patients in whom double-positive cells of each examined molecule were harvested. Graph II: Quantification of double-positive CTCs/total CTCs for each examined molecule. Graph III: Quantification of median expression per patient for each examined molecule. (B) Representative ARIOL system images of CTCs in PBMC cytospin. Row I: Cytospin double-stained with monoclonal pan-CK A45-B/B3 (green)/polyclonal Twist anti-rabbit (red) antibodies and DAPI nuclear staining. Original magnification, x400. Row II: Cytospin double-stained with monoclonal A45-B/B3 (green)/polyclonal vimentin anti-rabbit (red) antibodies and DAPI nuclear staining. Original magnification, x400. ARIOL system = automated image analysis system; CK = cytokeratin; CTCs = circulating tumour cells; PBMCs = peripheral blood mononuclear cells; DAPI = 4',6-diamidino-2-phenylindole.Back to article page