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Table 3 Ratio of mRNA for some PG genes to 18S RNA

From: Chondroitin sulfates play a major role in breast cancer metastasis: a role for CSPG4 and CHST11gene expression in forming surface P-selectin ligands in aggressive breast cancer cells

Antigen MCF7 MDA-MB-468 MDA-MB-231 MDA-MET
CSPG4 0.02 (± 0.006) 0.03 (± 0.02) 2.43 (± 0.56) 2.15 (± 0.8)
NRP-1 0.96 (± 0.10) 1.00 (± 0.08) 1.55 (± 0.20) 1.96 (± 0.07)
SDC-1 * 0.45 (± 0.07) 2.09 (± 0.18) 0.22 (± 0.03) 0.13 (± 0.04)
SDC-4 ** 0.58 (± 0.08) 0.86 (± 0.07) 1.50 (± 0.30) 1.90 (± 0.11)
ER1 4.84 (± 0.59) 0.12 (± 0.03) 0.14 (± 0.03) 0.11 (± 0.02)
  1. Gene expression was measured by qRT-PCR assays, repeated 3 times for each gene and each cell line and averages (± standard deviations) are shown. One-way ANOVA with Tukey's post-hoc procedure on log-transformed data was used for statistical analysis as described in Materials and Methods. Bold entries are statistically different (at P < 0.05) than non-bold entries in each row. *The expression of SDC-1 in MDA-MB-468 cells is significantly higher than that in MDA-MB-231 and MDA-MET cells. **The expression of SDC-4 in MDA-MB-468 cells is significantly less than that in MDA-MB-231 and MDA-MET cells. The expression of ER1 was used as a control knowing that MCF7 is ER-positive and the other three cell lines are ER-negative. CSPG4, Chondroitin Sulfate Proteoglycan 4; ER1, Estrogen Receptor 1; NPR-1: Neuropilin-1; SDC-1, Syndecan-1; SDC-4, Syndecan-4.