Antigen | MCF7 | MDA-MB-468 | MDA-MB-231 | MDA-MET |
|---|
CSPG4
| 0.02 (± 0.006) | 0.03 (± 0.02) | 2.43 (± 0.56)
| 2.15 (± 0.8)
|
NRP-1
| 0.96 (± 0.10) | 1.00 (± 0.08) | 1.55 (± 0.20)
| 1.96 (± 0.07)
|
SDC-1
*
| 0.45 (± 0.07) | 2.09 (± 0.18)
| 0.22 (± 0.03)
| 0.13 (± 0.04)
|
SDC-4
**
| 0.58 (± 0.08) | 0.86 (± 0.07)
| 1.50 (± 0.30)
| 1.90 (± 0.11)
|
ER1 | 4.84 (± 0.59)
| 0.12 (± 0.03) | 0.14 (± 0.03) | 0.11 (± 0.02) |
- Gene expression was measured by qRT-PCR assays, repeated 3 times for each gene and each cell line and averages (± standard deviations) are shown. One-way ANOVA with Tukey's post-hoc procedure on log-transformed data was used for statistical analysis as described in Materials and Methods. Bold entries are statistically different (at P < 0.05) than non-bold entries in each row. *The expression of SDC-1 in MDA-MB-468 cells is significantly higher than that in MDA-MB-231 and MDA-MET cells. **The expression of SDC-4 in MDA-MB-468 cells is significantly less than that in MDA-MB-231 and MDA-MET cells. The expression of ER1 was used as a control knowing that MCF7 is ER-positive and the other three cell lines are ER-negative. CSPG4, Chondroitin Sulfate Proteoglycan 4; ER1, Estrogen Receptor 1; NPR-1: Neuropilin-1; SDC-1, Syndecan-1; SDC-4, Syndecan-4.
