The key IGF-1R signaling components in the regulation of TAM resistance of MCF7/IGF-1R cells. (a) Selected kinase inhibitors inhibiting IGF-1R signal-mediated TAM resistance. (b) Selected siRNA targeting genes (as named) involved in IGF-1R signal-mediated TAM resistance. Concentrations used: TAM (1 μM), E2 (1 nM) and IGF-1 (100 ng/mL). (-), no kinase inhibitor. siCtrl, nontargeting siRNA. Results are representative of duplicate experiments. Data are expressed as means ± SD. *P < 0.05. **P < 0.01. ***P < 0.001. (c) MetaCore Pathway Analysis showing the functional activities of the key players (dashed circles) in IGF-1R signal-mediated TAM resistance. Fold changes in cell proliferation as the result of inhibition of selected kinase inhibitors or knockdown of target genes are given in Additional file 3. 1, experiment 1 (exp 1) with kinase inhibitors. 2, experiment 2 (exp 2) with siRNA. Red bar, increase in proliferation. Blue bar, decrease in proliferation.