Comparison of H2AFX status in MCF-7 vs. HeLa cells. (a) Real-time polymerase chain reaction analysis of gene copy number between the two cell lines using RNase P as an endogenous control. (b) Comparison of transcript expression using TaqMan assay for H2AX (Hs01573336_s1). (c) Confocal images of subcellular localization of H2AX and γ-H2AX before and after etoposide (10 μmol/l) treatment. (d) Fold difference in hsa-miR-24-2 expression using RNU 44 (Assay ID 001094; PN 4427975) for normalization. PI, propidium iodide.