During hormone depletion or tamoxifen treatment of breast cancer cells the estrogen receptor apoprotein supports cell cycling through the retinoic acid receptor α1 apoprotein

Table 2 Tamoxifen Insensitive genes Repressed by the Apo-ER -> Apo-RARα Axis

Gene	ATRA effect	RAR ^a binding <10 kb	Fold change siER	Fold change siRARα1	Gene	ATRA effect	RAR ^a binding <10 kb	Fold change siER	Fold change siRARα1
IFI44L	↓	+	1.69	2.22	UBL3	-	++	1.61	1.58
IFI44	↓	-	2.09	2.28	SLC7A11	-	++	1.71	2.03
CCPG1	↓	-	1.67	1.56	KIAA0652	-	++	1.57	1.63
SLC25A36	↓	-	1.66	1.73	PHLDB1	-	++	1.64	1.55
SETD5	↓	++	1.52	2.11	CCNT2	-	-	1.53	1.63
MALL	↓	-	1.67	1.81	STX3	-	-	1.52	1.59
LYPD1	↓	-	1.66	1.59	CLN8	-	-	1.71	1.72
FAM186A	↓	-	1.64	1.75	UBXN10	-	-	1.73	1.64
GPR158	↓	-	1.81	1.54	PVR	-	-	1.63	2.39
CDKN1A	↓	-	1.70	1.51	TNFRSF10A	-	-	1.54	1.62
CEACAM6	↑	+	2.35	1.63	FLJ31958	-	-	1.71	1.52
LGALS3BP	↑	-	1.98	1.92	C18orf25	-	-	1.50	1.61
CTSS	↑	-	1.50	1.86	TAPBP	-	-	1.62	1.51
SELL	↑	-	1.75	1.71	ADAM17	-	-	1.58	1.63
SHROOM1	↑	-	1.68	1.59	HCP5	-	-	1.59	2.52
CP	↑	++	1.71	1.75	DISC1	-	-	1.80	1.74
ABHD2	↑	++	1.56	1.57	SP100	-	-	1.77	1.61
ABLIM1	↑	++	1.56	1.55	AASS	-	-	1.53	2.17
ALOX5	↑	-	1.72	2.09	HLA-G	-	-	1.50	2.33
HLA-C	↑	-	1.51	2.51	HLA-B	-	-	1.61	2.08
MAP1B	↑	-	2.12	1.51	RSAD2	-	-	1.84	3.19
ARHGAP1	↑	-	1.58	1.55	SGSM3	-	-	1.72	1.50
SP110	↑	-	1.58	1.63	HLA-J	-	-	1.52	2.04
RTP4	↑	-	1.60	1.81	SESN1	-	-	1.53	1.60
MUC20	↑	-	1.75	1.91	ANO10	-	-	1.51	1.64
RNF38	-	+	1.80	1.69	SAMD9	-	-	1.68	1.56
C9orf80	-	+	1.64	1.53	FLJ13197	-	-	1.68	1.57
CEACAM5	-	-	2.35	1.70	PGLS	-	-	1.54	1.81
CNOT4	-	-	1.79	1.92	LMO3	-	-	1.61	1.58
PLXNA2	-	-	1.65	1.86	DNAJC21	-	-	1.68	1.61
TTC9	-	-	1.76	1.51	SETD2	-	-	1.52	2.11
EIF5A2	-	-	1.90	1.67	ZNF24	-	-	2.10	1.68
FLCN	-	++	1.51	1.53	TTLL11	-	-	1.72	1.51
BAZ2A	-	++	1.53	1.58	ZNF544	-	-	1.58	1.71

^a RAR binding site identified within 10 kb of the transcription start site (Hua et al., 2009).
++ RAR binding site detected under high stringency (Hua et al., 2009).
+ RAR binding site detected under low stringency (Hua et al., 2009).
↑ Up-regulated by ATRA (1 μM, 24 h) ≥2-fold.
↓ Down-regulated by ATRA (1 μM, 24 h) ≤50%.
Abbreviations: ER, estrogen receptor; RAR, retinoic acid receptor; ATRA, all trans-retinoic acid; si, small interfering RNA.

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