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Figure 5 | Breast Cancer Research

Figure 5

From: Cancer-related ectopic expression of the bone-related transcription factor RUNX2 in non-osseous metastatic tumor cells is linked to cell proliferation and motility

Figure 5

RUNX2 expression increases cell migration in non-metastatic MCF7 breast cancer cells. (A) Western blot analysis using a RUNX2 antibody and/or c-Myc epitope tag in MCF7 cells transfected with a Myc-RUNX2 expression vector. Levels of β-actin were used as a control for protein loading) (B) Images from a wound healing assay with MCF7 cells transfected with empty expression vector (pcDNA3 control) or a plasmid expressing Myc-RUNX2 at various time points (in hours). (C) Quantification of total percent closure of control MCF7 cells (pcDNA3; 34%) and Myc-RUNX2 expressing MCF7 cells (41%) revealed that RUNX2 expressing increases cell motility. Asterisk indicates statistical significance between Myc-RUNX2 and control treatment (P < 0.05 based on ANCOVA test). Error bars represent standard deviation.

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Breast Cancer Research

ISSN: 1465-542X

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