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Figure 4 | Breast Cancer Research

Figure 4

From: Cancer-related ectopic expression of the bone-related transcription factor RUNX2 in non-osseous metastatic tumor cells is linked to cell proliferation and motility

Figure 4

RUNX2 silencing reduces migration of metastatic MDA-MB-231 breast cancer cells. (A) Diminished expression of RUNX2 protein levels was observed by western blotting in lysates from MDA-MB-231 treated with siRUNX2. (B) Wound healing assay of MDA-MB-231 cells treated with siRUNX2 or a non-silencing RNA (AllStar Control) at five different time points. (C) Cell counts were performed to determine the number of cells migrating to the scratch zone after 24 h for MDA-MB-231 cells treated with siRUNX2 (n = 180 cells) or non-silencing AllStar Control (n = 242 cells). (D) Total percent closure was determined as a time-course after initiating the scratch. Control MDA-MB-231 cells transfected with AllStar Control (88%) achieved 29% percent closure, while cells treated with siRUNX2 (59%) exhibited reduced wound closure. Asterisk indicates statistical significance between siRUNX2 and control treatment (P < 0.01 based on ANCOVA test). Error bars represent standard deviation.

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Breast Cancer Research

ISSN: 1465-542X

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