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Figure 2 | Breast Cancer Research

Figure 2

From: A novel approach for the generation of genetically modified mammary epithelial cell cultures yields new insights into TGFβ signaling in the mammary gland

Figure 2

Morphology, proliferative capacity and intermediate filament expression of immortalized mammary epithelial cell cultures. (a) Western blot of tsTAg expression in immortalized mammary epithelial cells (IMECs) grown in the presence or absence of added IFNγ at permissive (33°C) or nonpermissive (37°C) temperatures as indicated. β-actin used as a loading control. (b) Phase contrast images of wildtype IMECs showing a cobblestone epithelial morphology under both permissive and nonpermissive conditions. White scale bar = 100 μm. (c) Proliferation of IMECs under the permissive and nonpermissive growth conditions, showing rapid loss of proliferative capacity under nonpermissive conditions. Results representative of two independent experiments. (d) Wildtype IMEC cultures were grown under nonpermissive conditions and the expression of intermediate filament proteins was assessed by immunofluorescence using pancytokeratin or vimentin antibodies. Swiss 3T3 fibroblasts were used as a positive control for vimentin expression. DAPI, 4',6-diamidino-2-phenylindole. (e) Flow cytometric analysis for expression of cytokeratins CK8 and CK14 in wildtype IMECs grown under nonpermissive conditions.

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Breast Cancer Research

ISSN: 1465-542X

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