Figure 1

Stat3 is required for the migratory, invasive and tumorigenic phenotype of Ras transformed MCF10A cells. (a) Extracts from Ras transformed MCF10A cells (MCF10A-Ras) expressing control (C) or Stat3 shRNA (S3sh) were analyzed for levels of Stat3, tyrosine phosphorylated (pStat3), Ras and Tubulin by Western blot analysis. (b) MCF10A-Ras cells expressing control (C) or Stat3 shRNA (S3sh) were plated in six-well dishes and cell numbers were determined daily for seven days. Each data point represents the mean value from triplicate wells. (c) MCF10A-Ras cells expressing control (C) or Stat3 shRNA (S3sh) were plated into Boyden chambers and cell migration (MIG) and invasion (INV) was determined with crystal violet staining after 24 hrs. Results are expressed as cells/field (mean ± SD of triplicates from three independent experiments). (d) Colony formation in soft agar of MCF10A-Ras cells expressing control (C) or Stat3 shRNA (S3sh) (mean ± SD of triplicates from three independent experiments). Tumor growth in nude mice using MCF10A-Ras cells expressing control (C) or Stat3 shRNA (S3sh) was determined (mean ± SD from four independent injections).