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Table 1 BRCA1/ 2 exon 11 primers and segments for high-throughput solid-phase protein truncation test

From: An ELISA-based high throughput protein truncation test for inherited breast cancer

Segment

Nucleotides

Segment size, base pairs

Primer sequence (5' to 3') F, forward; R, reverse; subscript 'I', intronic primer

BRCA1 a

   

1

123,126-125,033

1,908

F: gCTTgTgAATTTTCTgAgACggAT

   

R: TgTATTCTgCAAATACTgAgCATCAAg

2

124,617-126,578

1,962

F: gTCAATCCTAgCCTTCCAAgAgAA

   

RI: gggCAAACACAAAAACCTggTTCC

BRCA2 a

   

1

25,758-27,458

1,701

FI: TTTTgTCACTTTgTgTTTTTATgTTTAgg

   

R: gCCAgCAAACTTCCgTTTAATTTC

2

27,174-29,330

2,157

F: AACCATAATTTAACACCTAgCCAAAAg

   

R: TgAAgAgCTAgTCACAAgTTCCTC

3

29,181-30,743

1,563

F: gATTCTggTATTgAgCCAgTATTgAAg

   

RI: CACgAAAggTAAAAATgAACACTTACC

  1. aNational Center for Biotechnology Information (NCBI) reference sequences: Ng_005905.2 for BRCA1 and Ng_012772.1 for BRCA2, both numbered from whole sequence entry. BRCA1/BRCA2, breast and ovarian cancer susceptibility gene 1/2.
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Breast Cancer Research

ISSN: 1465-542X

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