Hyaluronidase increases the release rate of VEGF-C and ANG-2. Increasing levels of hyaluronidase activity (U/ml) is accompanied by increasing release of (a) VEGF-C or (b) ANG-2 from HAMC. Data are shown as means ± SEM (n = 3). Fifty units (red), 5 U (blue), 0.5 U (green), and no enzyme (black) are shown. Significant differences in ANG-2 release were reached by hour 24 in both the 50 U and 5 U enzyme concentration solutions. VEGF-C release did not reach significance. Significance in comparison to control levels (no HAase) was assessed by ANOVA and Dunnett's one-sided t-test and is indicated by asterisks. *P < 0.05.