Figure 7
From: Tamoxifen enhances the cytotoxic effects of nelfinavir in breast cancer cells
Nelfinavir has no effect on the chymotrypsin-like proteasome activity of breast cancer cells. A total of 5 Ă— 103 MCF7 and MDA-MB-435 cells per well were seeded in quadruplicate in 96-well cell culture dishes and were incubated for 5 hours with either 15 μg/ml nelfinavir (NFV) or 15 ng/ml bortezomib (BTZ). Chymotryptic proteasomal activity was analysed using the bioluminescent Promega Proteasome-Gloâ„¢ assay (Promega, Mannheim, Germany) with Suc-LLVY-aminoluciferin as a substrate. The BTZ-sensitive myeloic leukaemia cell line IM9 was used as a BTZ-responsive control and was treated with 8 μg/ml NFV and 4.5 ng/ml BTZ. Values represent the mean ± standard deviation. *Significance assumed at P < 0.05 with the nonparametric Mann-Whitney U rank-sum test. CON, control.