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Figure 7 | Breast Cancer Research

Figure 7

From: Tamoxifen enhances the cytotoxic effects of nelfinavir in breast cancer cells

Figure 7

Nelfinavir has no effect on the chymotrypsin-like proteasome activity of breast cancer cells. A total of 5 × 103 MCF7 and MDA-MB-435 cells per well were seeded in quadruplicate in 96-well cell culture dishes and were incubated for 5 hours with either 15 μg/ml nelfinavir (NFV) or 15 ng/ml bortezomib (BTZ). Chymotryptic proteasomal activity was analysed using the bioluminescent Promega Proteasome-Glo™ assay (Promega, Mannheim, Germany) with Suc-LLVY-aminoluciferin as a substrate. The BTZ-sensitive myeloic leukaemia cell line IM9 was used as a BTZ-responsive control and was treated with 8 μg/ml NFV and 4.5 ng/ml BTZ. Values represent the mean ± standard deviation. *Significance assumed at P < 0.05 with the nonparametric Mann-Whitney U rank-sum test. CON, control.

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