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Figure 3 | Breast Cancer Research

Figure 3

From: Trichostatin A enhances acetylation as well as protein stability of ERα through induction of p300 protein

Figure 3

p300 regulates acetylation and the protein level of ERα. (a) HeLa cells were transiently transfected with 2 μg Myc-ERα with or without 2 μg Myc-p300. Then 500 μg whole-cell lysate was immunoprecipitated (IP) using anti-acetylated-lysine (Ac-Lys) or normal IgG antibodies, and probed by anti-ERα antibody. The expression of proteins was analyzed by western blot (WB) using anti-Myc or anti-ERα antibody as input. (b) HeLa cells were transiently transfected with 2 μg Myc-ERα or the indicated amounts of Myc-p300 or empty vector. The expression of proteins was analyzed by WB using anti-ERα, anti-Myc or anti-p300 antibodies. (c) HeLa cells were transiently transfected with 4 μg Myc-ERα and treated with or without 1 μM trichostatin A (TSA) for 3 hours. Then 500 μg whole-cell lysate was immunoprecipitated using anti-p300 or normal IgG antibodies, and probed by anti-Myc antibody.

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