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Table 2 Intracellular concentrations (nmol/g cell weight) of 13 C-labeled endogenous metabolites (glycolysis and TCA cycle intermediates, glucose) and lipid metabolites (choline-containing phospholipids, cholesterol)

From: Effects of lovastatin on breast cancer cells: a proteo-metabonomic study

  Control Lovastatin lactone Lovastatin
acid
13 C-lactate 612 ± 36 252 ± 51** 343 ± 15**
glycolysis int 845 ± 21 369 ± 13*** 467 ± 52***
TCA cycle int 913 ± 232 189 ± 28** 213 ± 56**
glucose intracell 1918 ± 382 2691 ± 283* 2758 ± 231*
citrate 323 ± 46 98 ± 33*** 153 ± 35**
glutathione 2595 ± 168 871 ± 72*** 1149 ± 78**
choline-PL 3757 ± 534 2158 ± 275** 2672 ± 542*
chol C18+C19 3914 ± 582 2125 ± 289** 2467 ± 351**
  1. The values were calculated based on MDAMB468 cell extracts as assessed by 1H-NMR and 13C-NMR. The cells were incubated with 8 μg/mL lovastatin lactone or hydroxy acid for 48 hours. Values are presented as means ± standard deviation of three independent experiments. Significance levels: * P < 0.05; ** P < 0.005; *** P < 0.001 were determined by analysis of variance (with post-hoc pairwise multiple comparison Tukey-test). chol, cholesterol; choline-PL, choline-containing phospholipids; glu, glutamate; glycolysis int, glycolysis intermediates: 13C-lactate+13C-alanine, TCA int: TCA cycle intermediates: (C2 + C3 + C4)-glutamate + (C2 + C3)-glutamine.