Knockdown of PIPKIγ blocks breast cancer cell line migration and invasion. MDA-MB-231 or MDA-MB-435S cells were transfected with control siRNA, or type I gamma phosphatidylinositol phosphate kinase (PIPKIγ) specific siRNA separately as indicated. (a) Expression of PIPKIγ and actin were detected by their specific antibodies. (b) FBS (1%) induced migration of MDA-MB-231 and MDA-MB-435S cells. (c) FBS (1%) induced invasion of MDA-MB-231 and MDA-MB-435S cells.