Differential FOXC1 methylation across diagnosis groups and subsequent validation by qRT-PCR. A: Differences in FOXC1 average DNA methylation (%) between normal breast tissue and the different diagnosis groups. The FOXC1 average DNA methylation (%) value is the average value of methylation for all CpGs calculated for each tumour sample. B: Differences in relative expression levels of FOXC1 as measured by qRT-PCR in normal breast tissue versus methylated and unmethylated tumours. Expression of FOXC1 was measured relative to the expression of the reference gene PGK1. Black horizontal bars represent median value for each diagnosis group.