Figure 4From: The membrane mucin MUC4 is elevated in breast tumor lymph node metastases relative to matched primary tumors and confers aggressive properties to breast cancer cellsCharacterization of 1G8s for immunohistochemistry. (a) A375-Rep8 cells (24) were treated with or without tetracycline to induce Muc4 expression, and cells were harvested and lysates were blotted with 1G8s and anti-tubulin (left panels), or cells were fixed in formalin, embedded in paraffin and analyzed by immunohistochemistry using 1G8s. (b) MCF10A-hMUC4/Y cells were treated with or without tetracycline to induce Muc4 expression, lysed and blotted with 1G8s and anti-tubulin (left panels), or analyzed by immunohistochemistry using 1G8s. (c) JIMT-1 cells stably transduced with MUC4-directed shRNA (KD) or scramble (scr) were blotted with 1G8s and anti-tubulin (upper panels), or analyzed by immunohistochemistry using 1G8s.Back to article page