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Figure 4 | Breast Cancer Research

Figure 4

From: The membrane mucin MUC4 is elevated in breast tumor lymph node metastases relative to matched primary tumors and confers aggressive properties to breast cancer cells

Figure 4

Characterization of 1G8s for immunohistochemistry. (a) A375-Rep8 cells (24) were treated with or without tetracycline to induce Muc4 expression, and cells were harvested and lysates were blotted with 1G8s and anti-tubulin (left panels), or cells were fixed in formalin, embedded in paraffin and analyzed by immunohistochemistry using 1G8s. (b) MCF10A-hMUC4/Y cells were treated with or without tetracycline to induce Muc4 expression, lysed and blotted with 1G8s and anti-tubulin (left panels), or analyzed by immunohistochemistry using 1G8s. (c) JIMT-1 cells stably transduced with MUC4-directed shRNA (KD) or scramble (scr) were blotted with 1G8s and anti-tubulin (upper panels), or analyzed by immunohistochemistry using 1G8s.

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