Table 1 Antibody characterization, dilutions and positive controls
From: Immunohistochemical characterization of subtypes of male breast carcinoma
Antigen | Vendors | Species (clone) | Dilution | Positive control |
|---|---|---|---|---|
ER | Novocastra Lab Ltd. Newcastle, UK | Mouse IgG1 (6F11) | 1:50 | Breast |
PR | NeoMarkers Inc. Fremont, CA, USA | Mouse IgG1 (1A6) | 1:30 | Breast |
HER2 | NeoMarkers Inc. Fremont, CA, USA | Mouse IgG1 (e2-4001) | 1:100 | Breast carcinoma |
CK 5/6 | Deko Inc, Carpinteria, CA, USA | Mouse IgG1 kappa (D5/16 B4) | 1:100 | Skin |
EGFR | Zymed Lab Inc. San Francisco, CA, USA | Mouse IgG1 (31G7) | 1:50 | Squamous cell carcinoma |
NF-κB p56 | Abcam Inc. Cambridge, MA, USA | Rabbit polyclonal IgG | 1:80 | Large B cell lymphoma |