Expression of Wnt regulated genes in HC11 stem cell-like, pre-differentiated and differentiated cells. Wnt regulated gene expression evaluated by western blot and immunofluorescence in HC11 stem cell-like cells, pre-differentiated cells and differentiated cells. Top panel, Birc5; bottom panel, c-myc. In all cases, whole cell extracts were resolved on 12% SDS-PAGE and membranes were blotted with the indicated antibodies. Tubulin was used as loading control.