Figure 2
Absence of Wnt5a enhances growth of MMTV-PyVmT tumours. (a) Representative bright field images of H&E-stained MMTV-PyVmT and MMTV-PyVmT:Wnt5a-/- tumours. Arrows denote the large pleomorphic nuclei in Wnt5a-/- tumours. (b) Tumour volume at the time of sacrifice. Tumour volume is significantly larger in the absence of Wnt5a (eight per group, PyVmT mean tumour volume 0.46 cm3, PyVmT:Wnt5a-/- mean tumour volume 1.1 cm3; P < 0.0001). (c) Proliferation of MMTV-PyVmT:Wnt5a-/- and control MMTV-PyVmT tumours. Ki67 staining illustrates a significant increase in proliferation in the absence of Wnt5a (P < 0.0001, eight MMTV-PyVmT:Wnt5a-/- sections, three fields per section; four MMTV:PyVmT sections, three fields per section). DNIIR = dominant-negative transforming growth factor-β type II receptor; H&E = haematoxylin and eosin; MMTV = mouse mammary tumour virus promoter/enhancer; PyVmT = polyoma virus middle T antigen; Wnt = Wingless-related protein family.