Table 1 Identification of the seven protein spots with liquid chromatography-tandem mass spectrometry
Spot number | Protein symbol | Theoretical MW (kDa) | Experimental MW (kDa) | Theoretical pI | Experimental pI | Ratio (MCF-7-MEK5 versus MCF-7-VEC) |
|---|---|---|---|---|---|---|
0415 | Keratin 19 (KRT19) | 44.07 | 46 | 4.9 | 5.2 | 0.01 |
1207 | Glutathione-S-transferase Mu 3 (GSTM3) | 26.54 | 26 | 5.25 | 5.7 | 0.37 |
1601 | Vimentin (VIM) | 53.62 | 55 | 4.91 | 5.2 | 2.14 |
1806 | Heat shock 70 kDa protein 4 (HSPA4) | 94.24 | 100 | 5.03 | 5.3 | 1.81 |
2110 | Glutathione-S-transferase P (GSTP1) | 23.34 | 23 | 5.32 | 5.8 | 5.41 |
2403 | Creatine kinase B-type (CKB) | 42.62 | 45 | 5.25 | 5.7 | 57.67 |
2503 | Keratin 8 (KRT8) | 53.67 | 50 | 5.38 | 5.8 | 0 |