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Figure 5 | Breast Cancer Research

Figure 5

From: Proteomic analysis of tumor necrosis factor-α resistant human breast cancer cells reveals a MEK5/Erk5-mediated epithelial-mesenchymal transition phenotype

Figure 5

The differences in genes involved in EMT in MCF-7-MEK5 and MCF-7-VEC cells. (a) With RT-PCR assay, the genes slug, δ-ef1, and β-catenin were upregulated, whereas E-cadherin and snai1 were downregulated in MCF-7-MEK5 cells compared with MCF-7-VEC cells. (b) With real-time PCR, relative expression levels for N-cadherin, E-cadherin, β-catenin, and snail were analyzed in MCF-7-VEC and MCF-7-MEK5 cells. The gene actb was used as an internal control. (c) MCF-7, MCF-7-TNR, MCF-7F, and MCF-7-MEK5 cells were analyzed for expression of SNAI2 (slug) or ZEB1 (δEF-1) by real-time PCR. Expression was normalized to MCF-7 cells, with actb used as an internal control. MEK, mitogen-activated protein kinase kinase.

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Breast Cancer Research

ISSN: 1465-542X

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