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Figure 4 | Breast Cancer Research

Figure 4

From: Proteomic analysis of tumor necrosis factor-α resistant human breast cancer cells reveals a MEK5/Erk5-mediated epithelial-mesenchymal transition phenotype

Figure 4

Differences in vim , krt8 , krt19 , hspa4 , gstp1 , gstm3 , and ckb between MCF-7-MEK5 and MCF-7-VEC cells. (a) Regular RT-PCR assay. (b) Real-time PCR assay was used to quantitate relative mRNA expression between MCF-7-VEC and MCF-7-MEK5 cells. The normalized expression folds in MCF-7-MEK5 cells were 814.15 ± 145.23 (vim), 0.01 ± 0.00 (krt8), 0.00 ± 0.00 (krt19, undetected), 1.51 ± 0.56 (hspa4), 40,637.45 ± 15,815.03 (gstp1), 0.13 ± 0.01 (gstm3), and 38.59 ± 7.87 (ckb) compared with MCF-7-VEC cells. The gene actb was used as an internal control. MEK, mitogen-activated protein kinase kinase.

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Breast Cancer Research

ISSN: 1465-542X

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