Figure 3

Lower phosphatase and tensin homolog deleted on chromosome 10 (PTEN) expression in human basal-like cancers (BLCs) compared with human epidermal growth factor receptor overexpressing (HER2+) carcinomas. (a) Lower PTEN protein levels in BLCs compared with HER2+ carcinomas. PTEN protein level was quantified by reverse phase protein array (RPPA). Outliers are shown for BLCs (solid circles) and HER2+ carcinomas (open circles). Data are representative of four separate experiments. (b) Lower mRNA PTEN level (probeset 225363_at) in BLCs compared with HER2+ carcinomas. An outlier is present in BLC population (solid circles). (c) Correlation between PTEN protein measured by RPPA and PTEN messenger in the entire tumour population. Linear regression, Spearman correlation c and p value are presented. BLCs (solid circles) and HER2+ carcinomas (open circles) are shown. (d) Stathmin is overexpressed in BLCs compared with HER2+ carcinomas. Box plots indicate the levels of stathmin protein measured by RPPA within the two populations. Data are representative of four separate experiments. P values are shown (a,b,d: Mann-Whitney test). * p < 0.05, ** p < 0.01, *** p < 0.001. Protein and mRNA relative quantifications were logarithmic transformed.