Figure 1
From: PAI-1 and functional blockade of SNAI1 in breast cancer cell migration
Immunofluorescence analysis of E-cadherin (green) in MDA-mock and SNAI1-DN cells. Nuclei are visualised by 4', 6-diamidino-2-phenylindole (DAPI) stain. (a) The MDA-mock control cell line transfected with a control vector (mock) presents no cell-cell contact, and the immunostaining of E-cadherin is very low and diffused. (b 1 ) SNAI1-DN cells have acquired an epithelial like phenotype with E-cadherin stronger expression (b 2 ) sometimes organised specifically at cell-cell contacts. (c) Semi-quantitative RT-PCR analysis of E-cadherin mRNA in MDA-mock and SNAI1-DN cells. GADPH mRNA levels were detected as a control of cDNA inputs. SNAI1-DN expression in MDA-MB-231 cells lead to the re-expression of E-cadherin mRNA.