S100A4 protein is reduced in MDA/RLN2 nude mouse xenografts. (a) Total RNA was extracted from nude mice tumours and reverse transcriptase polymerase chain reaction (RT-PCR) analysis was performed for human RLN2. Representative results are shown for three tumours grown in mice injected with MDA/RLN2 transfectants (R-T1, R-T2, R-T3) and three tumours from mice injected with MDA/EGFP transfectants (E-T1, E-T2, E-T4). Mouse liver was used as negative control tissue. 18S transcript levels were used as reference. All MDA/RLN2 xenografts contained RLN2 transcripts. (b) A representative Western analysis is shown for four MDA/RLN2 tumours (R-T1, R-T2, R-T3, R-T4) demonstrating the down-regulation of S100A4 in all tumours when compared with tumours derived from MDA/EGFP transfectants (E-T1, E-T2). Beta-actin was used to control for protein loading of the gel.