Figure 1

Abundance of the expression of ISG15 mRNA in breast cancer cell lines. Semiquantitative real-time PCR (LightCycler) of ISG15 expression was performed on reverse transcribed RNA from non-malignant (white bars) and malignant cell lines (black bars). ISG15 mRNA expression was low among the non-malignant mammary epithelial cell lines HMEC and MCF12A, whereas ISG15 mRNA transcript levels varied more and in summary were elevated in the investigated cancerous cell lines.