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Figure 4 | Breast Cancer Research

Figure 4

From: The aromatase inhibitor letrozole and inhibitors of insulin-like growth factor I receptor synergistically induce apoptosis in in vitro models of estrogen-dependent breast cancer

Figure 4

Letrozole and AEW541 exhibit synergistic proapoptotic activity at suboptimal concentrations. (a) Steroid-deprived MCF7/Aro and T47D/Aro cells were treated for 96 hours with either no Δ4A (-Δ4A), Δ4A in the presence of dimethyl sulfoxide (DMSO; +Δ4A), or Δ4A in the presence of the indicated concentrations of compound. Cells were processed for fluoresence-activated cell sorting (FACS) using propidium iodide as described. Percentage of cells in sub-G1 is indicated. (b) Steroid-deprived MCF7/Aro cells were treated for 96 hours with either no Δ4A (-Δ4A), Δ4A in the presence of DMSO (+Δ4A), or Δ4A in the presence of the indicated concentrations of compound. Cells were processed for FACS using 7-AAD/Annexin V staining and apoptotic subpopulations identified as described in the Materials and methods section. Note that the apoptotic population resides in the upper and lower right panels of the 7-AAD/Annexin V stained cells (circled by the dotted line). PI, propidium iodide.

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Breast Cancer Research

ISSN: 1465-542X

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