Figure 5

JS-K increases TIMP-2 production to suppress breast cancer invasion through Matrigel. (a) Expression of matrix metalloproteinases (MMPs) and tissue inhibitor of matrix metalloproteinases (TIMPs) in the conditioned medium supernatant obtained from untreated and JS-K-treated cells was qualitatively screened using a human MMP array kit. (b) TIMP-2 levels in the conditioned medium supernatant obtained from untreated and JS-K-treated cells were determined using a commercial ELISA kit. Columns indicate the mean of triplicate wells ± standard deviation. *Significant increase in the TIMP-2 levels relative to untreated cells, P < 0.05. (c) MDA-MB-231, F10, and MCF-7/COX-2 cells were treated with JS-K (1 μM) in the presence or absence of a TIMP-2 neutralizing antibody (2.5 μg/ml) for 72 hours in a Matrigel invasion assay. (d) The number of invaded cells was counted. Columns indicate the mean of triplicate wells ± standard deviation. *Significant decrease in the number of invaded cells relative to untreated cells, P < 0.05. ^†Increase relative to JS-K-treated cells, P < 0.05.