Eupatorin causes G2/M arrest in MDA-MB-468 cells, whereas this effect is not observed in MCF-10A cells. (a) Flow cytometric DNA analysis of MDA-MB-468 and MCF-10A cells treated with 10 μM eupatorin. (b) Percentage of MDA-MB-468 cells in G1, S+G2/M, and sub-G1 phases of the cell cycle. Cells were plated in 24-well plates and left to grow for 48 hours. Eupatorin was incubated with the cells for 30 and 48 hours, and 0.1% dimethylsulfoxide was used as a control. The cells were stained with propidium iodide and analysed using a Beckman Coulter flow cytometer as described in Materials and methods. Error bars represent mean ± standard deviation for n = 3 determinations.