Methylation analysis of BRCA1 in KCF3. (a) MethyLight results for BRCA1 and control gene HMBS for samples from KCF3. The BRCA1 MethyLight assay indicates the presence of methylated DNA for the peripheral blood leukocyte (PBL), buccal mucosa (BUC) and tumour (TUM) of KCF3. The HMBS control gene indicates the amount of bisulfite modified DNA for each sample of KCF3. (b) Methylation-sensitive high resolution melting (MS-HRM) results for samples from KCF3. The PBL sample (pink curve) and the BUC sample (green curve) show methylation levels close to the 10% methylated control. The TUM sample (orange curve) shows a much higher level of methylation.