Figure 1

Immunohistochemical staining of breast carcinoma with polyclonal antibody against derlin-1. (a) Positive derlin-1 labeling in a breast cancer section. Note that the immunoreactivity was limited to the cytoplasm of the neoplastic cells. (b) Negative derlin-1 labeling in the section from the same breast cancer tissue block when derlin-1 antibody was pre-incubated with the corresponding peptide antigen. (c) The absence of signal was scored as '0'. (d) Low-intensity signal was scored as '1'. (e) Moderate-intensity signal was scored as '2'. (f) High-intensity signal was scored as '3'. Bars, 50 μm.