Distribution of pAkt expression. (a) pAkt level determined by immunohistochemistry (IHC) was quantified using DigiPro software (Labomed, Inc., Culver City, CA, USA) according to the percentage of tumor area with positive pAkt stain as mentioned in Materials and methods. The x-axis indicates the percentage of tumor area with positive stain. The range of the percentage of tumor area stained positive for pAkt was from 0% (negative stain) to 85% and was plotted at 5% intervals. The y-axis indicates the total number of cases at each of the 5% intervals. Each bar represents the total number of cases within the indicated percentage of tumor area which was positively stained by pAkt. (b) The box plot describes the distribution of pAkt index in 141 patients. The level of pAkt determined by IHC and quantified with DigiPro software represents pAkt index as stated in Materials and methods (pAkt index = intensity × percentage of tumor area with positive stain). The range of pAkt index in the 141 patients is described by the box plot. The median level of pAkt index was 36. The value 255 was one outlier.