Figure 2
From: Silencing of GM3 synthase suppresses lung metastasis of murine breast cancer cells
Analysis of ganglioside content on cell surface by flow cytometry. Expression levels of (a) GM3 and (b) GD3 gangliosides on the surface of GM3S-silenced 4T1 cells using anti-GM3 and anti-GD3 monoclonal antibody (shaded peaks: 4T1-siGM3S2; unshaded peaks: 4T1-siMock). Expression levels of (c) GM3 and (d) GD3 gangliosides on the surface of GM3S over-expressing 67NR cells using anti-GM3 and anti-GD3 monoclonal antibody (shaded peaks: 67NR-Control; unshaded peaks: 67NR-GM3S). CMP, cytidine 5'-monophosphate; GM3S, CMP-N-acetylneuraminic acid:lactosylceramide 2,3-sialyltransferase.