Densitometric analysis of leptin signaling protein and mRNA expression levels in mammary fat pad (MFP) from Low-Fat or High-Fat mice. (a) Leptin signaling proteins in MFP. Protein bands were obtained using Western blot analysis as described in Materials and methods. Data are from five Low-Fat and four High-Fat mice. (b) Average densitometry of leptin signaling molecule mRNA expression levels in MFP. Average density values of the mRNA expression bands were from five Low-Fat and six High-Fat mice. *p < 0.05. HF, High-Fat; Jak2, Janus kinase 2; LF, Low-Fat; pSTAT3, phosphorylated signal transducer and activator of transcription 3; STAT3, signal transducer and activator of transcription 3.