Densitometric analysis of leptin signaling protein and mRNA expression levels in mammary tumor (MT) of Low-Fat and High-Fat mice. (a) Leptin signaling protein expression in MT. (b) Leeptin mRNA expression levels in MT. Protein bands were obtained using Western blot analysis as described in Materials and methods. Results are the average of three and six MT samples of individual mice. *p < 0.05. HF, High-Fat; Jak2, Janus kinase 2; LF, Low-Fat; pSTAT3, phosphorylated signal transducer and activator of transcription 3; STAT3, signal transducer and activator of transcription 3.