Figure 2

Transformation of HGFIN knock-out MCF12A. (a) Representative colonies in 5-day clonogenic assays in methylcellulose with MCF-12A as untransfected, stably transfected with pPMSKH1, or pPMSKH1-HGFIN siRNA. (b) The total number of colonies in methylcellulose cultures, plated with 100 cells/dish are presented as mean ± SD, n = 5. (c) Growth curves were established with MCF-12A as untransfected or stably transfected pPMSKH1 or pPMSKH1-HGFIN siRNA. The total numbers of viable cells were counted at weekly intervals and the results presented as mean ± SD, n = 5. *p < 0.05 vs culture with pPMSKH1-HGFIN siRNA.